Fig. 9.

R269C-ER enhances migration of pancreatic cancer cells. COLO-357 (a) PANC-1 (b) and MCF-7 (c) were seeded in 6-well plates and transfected with either WT-ER, R269C-ER or pcDNA3 constructs. Cells were grown in estrogen-depleted medium and the monolayer was scraped in a straight line, then treated with E2 (10nM) or a vehicle control for 48 h. The results are from a representative experiment of at least three independent experiments. Mean values of at least 10 measurements for each time point and condition are shown on the graphs and representative photos are also presented, *, P < 0.05, **, P < 0.01