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. 2020 Jun 8;20:178. doi: 10.1186/s12906-020-02976-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Betulinic acid inhibits PANC-1 and SW1990 cells proliferation. CCK8 assay of PANC-1(a) and SW1990 (c) cells incubated with 5 μM,10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 90 μM. Betulinic acid or an equal volume of DMEM medium for 24 h. Label-free Real-time Cellular Analysis (RTCA) following PANC-1 (b) and SW1990 (d) cells incubated with betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h. (e) Ki67 Immunofluorescence following PANC-1 and SW1990 cells incubated with betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h