Fig. 2.

This figure shows (A) the experimental casting protocol in which the hindlimb of Pax7-DTA mice were immobilized in plantarflexion for 2 weeks to reduce the number of serial sarcomeres in the soleus muscle. The mice were treated with oral gavage for 5 days with tamoxifen or a vehicle control. Subsequently, the mice were allowed to recover for 1 month and their muscles were harvested. (B) This is a representative image from the fluorescence-activated cell sorting analysis of the gastrocnemius and quadriceps muscles to quantify satellite cells; that is, α-7 integrin⁺/neural cell adhesion molecule⁺/CD31^-/CD45^- cells in the tamoxifen-treated (right) and vehicle-treated groups (left). The blue boxes indicate the representative cell populations. (C) The number of satellite cells was quantified with a fluorescence-activated cell sorting analysis, and this image shows substantial knockdown in satellite cells in mice in the tamoxifen-treated group (right) compared with those in the vehicle-treated control group. Data are shown as the mean ± SD.