Effects of c-Met TKI and Hsp90 inhibitor on cell signaling and proliferation of MKN45 cells. (A) MKN45 cells were treated with SU11274 (2.5 μM) or 17-AAG (0.5 μM) for the indicated times, and analyzed by immunoblotting using specific antibodies. (B) MKN45 cells were treated with SU11274 for 4 h (lane 2) or 96 h (lane 3–5). Rottlerin (10 μM) was added for 4 h (lane 4) or 96 h (lane 5). Cell lysates were analyzed by immunobloting with the indicated antibodies. (C) MKN45 cells were incubated continuously with DMSO (◆), SU11274 (▴), 17-AAG (◻) or SU11274 and rottlerin (*), and cell proliferation was monitored by MTS assay. Each point represents the mean of 4 determinations; error bars, SD.