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. Author manuscript; available in PMC: 2020 Jun 9.
Published in final edited form as: Mol Cancer Ther. 2009 Jul 7;8(7):2007–2014. doi: 10.1158/1535-7163.MCT-09-0336

Figure 4. γ-H2AX foci induced by PM00104 are independent of NER and are replication-associated.

Figure 4.

A and B, γ-H2AX foci in XPD and XPD-c cells treated with 10 nM Et743 or PM00104 for 6 hours. A, Representative pictures. B, Fold increase of the intensity of γ-H2AX staining normalized to non-treated cells (AU, arbitrary units). Values are averages of five independent experiments. Bars are SEM. C and D, Inhibition of PM00104-induced γ-H2AX by the DNA polymerase inhibitor, aphidicolin (APD). XPD-c cells were pre-incubated with 1μM APD for 15 minutes. PM00104 (10 nM) was then added for 6 additional hours. C, Representative pictures. D, Fold increase of the intensity of γ-H2AX staining normalized to untreated cells (AU, arbitrary units). Values are the average of three independent experiments. Bars are SEM.