Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | Anti- digoxigenin-POD, sheep, polyclonal Fab fragments | Sigma-Aldrich, Rouche | Roche, Cat# 11207733910, RRID:AB_514500 | 1:3000 |
Sequence-based reagent | cFos _F | This paper | PCR primers | CCGATACACTGCAAGCTGAA |
Sequence-based reagent | cFos_R | This paper | PCR primers | ATTGCAGGGCTATGGAAGTG |
Peptide, recombinant protein | Proteinase K | Sigma-Aldrich | Cat# P6556-10MG | 2 mg/ml |
Commercial assay | TSA Plus Cyanine three system | Sigma-Aldrich, Perkin Elmer | Cat# NEL74401KT | Dilution 1:50 |
Chemical compound, drug | Buspirone hydrochloride | Sigma-Aldrich | Cat# B7148-1G | 30 uM and 50 uM |
Software, algorithm | Anaconda, Spyder | Anaconda (https://www.anaconda.com/) | Spyder, RRID:SCR_017585 | Version 4.0.1 |
Software | ImageJ | NIH (http://imagej.nih.gov/ij/) |
RRID:SCR_003070 | |
Software | ANTs- Advanced Normalisation Tools | http://stnava.github.io/ANTs/ | RRID:SCR_004757 | Version 2.1.0 |
Other | DAPI staining | Sigma-Aldrich | Cat# D9564-10MG |
1 mg/ml |
Other | Slc6a4b RNA probe | Norton et al., 2008 | ||
Other | DAT RNA probe | Filippi et al., 2010 | ||
Other | Th1 RNA Probe | Filippi et al., 2010 | ||
Other | Th2 RNA probe | Filippi et al., 2010 |