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. 2020 Jun 9;9:e53370. doi: 10.7554/eLife.53370

Figure 4. Functional cross-activity between the A. japonicus and vertebrate Kisspeptin/Kisspeptin receptor systems.

Figure 4.

Intracellular Ca2+ mobilization in AjKissR1- (A) or AjKissR2-expressing (B) HEK293 cells was measured in response to 1.0 μM DrKiss1-10, DrKiss2-10, XtKiss1b-10, or HsKiss1-10 using Fura-2/AM. No Ca2+-mobilization-mediated activity was detected in AjKissR1- (C) or AjKissR2-expressing (D) HEK293 cells upon administration of the indicated concentrations of human neuropeptide S (HsNPS). Intracellular Ca2+ mobilization in human kisspeptin receptor (HsKiss1R)-expressing HEK293 cells was measured in response to 1.0 μM HsKiss1-10, AjKiss1a or AjKiss1b (E), as well as in zebrafish kisspeptin receptor (DrKiss1Ra or DrKiss1Rb)-expressing cells responding to 1.0 μM DrKiss1-10, AjKiss1a, or AjKiss1b (F, G). Figure 4—source data 1 shows the primary metadata. All data shown are representative of at least three independent experiments.

Figure 4—source data 1. Primary metadata of Ca2+ mobilization assay for Figure 4A-G.