Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens) | HEK293 (Human embryonic kidney293 cells) | American Type Culture Collection | ||
| Cell line (M. musculus) | STHdhQ7; STHdhQ111(mouse striatal neuronal progenitor cells) | Dr M Macdonald (Center for Genomic Medicine, Boston, USA) | ||
| Strain, strain background (Mus musculus) | HdhQ7/Q111; HdhQ7/Q7 | Dr M Macdonald (Center for Genomic Medicine, Boston, USA) |
HdhQ111
MGI:1861935 |
|
| Strain, strain background (Mus musculus) | R6/1; R6/2 | The Jackson Laboratory (Bar Harbor, ME, USA) | R6/1: MGI:2389466 For R6/2: MGI:2386951 |
|
| Strain, strain background (H. sapiens) | Post-mortem human brain sections containing caudate-putamen | Tissue Bank at Hospital Universitario Fundación Alcorcón (Madrid, Spain) Netherlands Brain Bank (Amsterdam, The Netherlands) |
For details and characteristics of human samples see: “Moreno E., et al., Neuropsychopharmacology. 2018 PMID:28102227’ |
|
| Antibody | anti-D1R (guinea pig) | Frontier Institute | Cat. # D-1R-GP-Af500 RRID:AB_2571595 |
Dilution: 1/200; 1/100 |
| Antibody | anti-H3R (rabbit polyclonal) | Alpha diagnostic | Cat. # H3R31-A RRID:AB_1617140 |
Dilution: 1/200 |
| Antibody | goat Alexa Fluor 488 anti-guinea pig antibody | Jackson Immunoresearch Laboratories | Cat. #106-545-003 RRID:AB_2337438 |
Dilution: 1/100 |
| Antibody | anti-phospho-p38 MAPK (Thr180/Tyr182) (rabbit polyclonal) | Cell Signaling | Cat. #9211S RRID:AB_331641 |
Dilution: 1/1,000 |
| Antibody | anti-β-tubulin (mouse monoclonal) | Sigma | Cat# SAB4200715 RRID:AB_2827403 |
Dilution: 1/10,000 |
| Antibody | IRDye 680 goat anti-rabbit antibody | Li-cor | Cat. #926–68071 RRID:AB_10956166 |
Dilution: 1/10,000 |
| Antibody | IRDye 800 goat anti-mouse antibody | Li-cor | Cat. # 926–32210 RRID:AB_621842 |
Dilution: 1/10,000 |
| Antibody | anti-spinophilin (rabbit polyclonal) | Millipore | Cat# 06–852 RRID:AB_310266 |
Dilution: 1/250 |
| Antibody | Cy3 anti-rabbit secondary antibodies | Jackson ImmunoResearch Laboratories |
Cat# 111-165-003 RRID:AB_2338000 |
Dilution: 1/200 |
| Antibody | Anti-1C2 (mouse monoclonal) |
Millipore | Cat# MAB1574 RRID:AB_94263 |
Dilution: 1/1,000 |
| Recombinant DNA reagent | Clone V3LHS_638095 | Thermo Scientific | ||
| Recombinant DNA reagent | Clone V3LHS_638091 | Thermo Scientific | ||
| Recombinant DNA reagent | psPAX2 | Addgene#12260 | ||
| Recombinant DNA reagent | pMD2.G | Addgene#12259 | ||
| Recombinant DNA reagent | RHS4346 | Thermo Scientific | ||
| Recombinant DNA reagent | H3R-shRNA and control-shRNA | This study | See Materials and methods | |
| Sequence-based reagent | RT-qPCR primers | This study | See Materials and methods | |
| Peptide, recombinant protein | TAT-TM peptides | This study | See Materials and methods | |
| Commercial assay or kit | Duolink II in situ PLA detection reagent red Kit | Sigma | Cat. #DUO92008 | |
| Commercial assay or kit | Duolink II PLA probe anti-guinea pig minus | Sigma | Cat. #DUO92010 | |
| Commercial assay or kit | Duolink II PLA probe anti-rabbit plus | Sigma | Cat. #DUO92002 RRID:AB_2810940 |
|
| Commercial assay or kit | High Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat. #4368814 | |
| Commercial assay or kit | Amplified Luminiscent Proximity Homogeneous Assay kit | AlphaScreen SureFire p‐ERK 1/2 (Thr202/Tyr204) Assay Kits PerkinElmer | Cat. # TGRESB | |
| Commercial assay or kit | [3H] SCH 23390 | PerkinElmer | Cat. # NET930 | 0.02 nM to 10 nM |
| Commercial assay or kit | [3H] R-α-methyl histamine | Perkinelmer | Cat. # NET1027 | 0.1 nM to 20 nM |
| Commercial assay or kit | SB 203580 | Tocris | Cat. # 1402 | 1 μM; 10 μM (see Materials and methods) |
| Commercial assay or kit | SKF 81297 | Tocris | Cat. # 1447 | 100 nM; 1 μM; 30 μM; 50 μM (see Materials and methods) |
| Commercial assay or kit | SCH 23390 | Tocris | Cat. # 0925 | one to 50 μM(see Materials and methods) |
| Commercial assay or kit | Thioperamide maleate salt | Sigma-Aldrich | Cat. #T123 | 10 μM (cells) 10 mg/kg (mice) |
| Software, algorithm | Grafit | Erithacus (http://www.erithacus.com/grafit/) |
||
| Software, algorithm | ImageJ | ImageJ (https://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software, algorithm | SMART junior | Panlab (http://www.panlab.com/panlabWeb/Software/php/displaySoft.php?nameSoft=SMART JUNIOR) |
RRID:SCR_012154 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism (https://www.graphpad.com/) | RRID:SCR_015807 | Version 6 |