Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Torulaspora delbrueckii) | FBA1 | Gordon et al., 2011 | TDEL0B06660; NCBI: XM_003679958 | Strain CBS1146; TdFBA1-S allele |
| Strain, strain background (Saccharomyces cerevisiae) | IMX585 | Mans et al., 2015 | S. cerevisiae strain with integrated CAS9 gene | |
| Strain, strain background (Torulaspora delbrueckii) | L09; L10; L11; L12; L13; L15; L16; L18; L19; L20 | This paper | Strain collection of Lallemand, Inc | |
| Strain, strain background (Torulaspora delbrueckii) | NCYC696 | National Collection of Yeast Cultures (UK) | ||
| Strain, strain background (multiple Torulaspora species) | All CBS strains | Westerdijk Fungal Biodiversity Institute (Netherlands) | ||
| Strain, strain background (Torulaspora pretoriensis) | UWOPS 83–1046.2 | MA Lachance, University of Western Ontario (Canada) | ||
| Strain, strain background (Zygotorulaspora mrakii) | NRRL Y-6702 | CP Kurtzman, USDA Agricultural Research Service | ||
| Strain, strain background (Naumovozyma castellii) | Y056 (NRRL Y-12630T); Y174 (CBS4310); Y287 (CBS3006); Y668 (CBS1579) | Jure Piškur, Lund University (Sweden) | See Spírek et al., 2003 | |
| Recombinant DNA reagent | pMEL13 plasmid | Mans et al., 2015 | ||
| Sequence-based reagent | ADE2.Y sgRNA template | DiCarlo et al., 2013 | ||
| Recombinant DNA reagent | pIL75-KanMX plasmid | Liachko and Dunham, 2014 | ||
| Recombinant DNA reagent | pWHO6 | This paper | DNA sequence is in Supplementary file 3 | |
| Recombinant DNA reagent | pWHO6-HA | This paper | DNA sequence is in Supplementary file 3 | |
| Recombinant DNA reagent | pGFP-HA | This paper | DNA sequence is in Supplementary file 3 | |
| Sequence-based reagent | Sc ade2::TdFBA1-S repair template | This paper | DNA sequence is in Supplementary file 3 | |
| Sequence-based reagent | Sc ade2::TdFBA1-R repair template | This paper | DNA sequence is in Supplementary file 3 | |
| Sequence-based reagent | Primers for PCR of Sc ade2::TdFBA1 locus | This paper | 5’-TGACCACGTT AATGGCTCC-3’ and 5’-CACCAGCTCCA GCGATAATTG-3’ | |
| Software, algorithm | SPAdes assembler | Bankevich et al., 2012 | V3.11.1 | RRID:SCR_000131 |
| Software, algorithm | HGAP3 assembler | Chin et al., 2013 |