Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (mouse) | CD1 | Harlan/Envigo | Cat#: 030 | |
| Genetic reagent (mouse) | Nkx2-1fl/fl | Kusakabe et al., 2006 | MGI: 3653645 | |
| Genetic reagent (mouse) | Sox2fl/fl | Shaham et al., 2009 | MGI: 4366453 | |
| Genetic reagent (mouse) | Tmem163Tg(Actincre) | Lewandoski et al., 1997 | MGI: 2176050 | |
| Genetic reagent (mouse) | Nkx2.5cre | Stanley et al., 2004 | MGI: 2448972 | |
| Antibody | α-NKX2-1 (rabbit polyconal) | Millipore | Cat#: 07601 | 1:200 for IF, 5ug for ChIP |
| Antibody | α-SOX2 (goat polyclonal) | Neuromics | Cat#: GT15098 | 1:250 for IF |
| Antibody | α-SOX9 (Rabbit polyclonal) | Santa Cruz | Cat#: sc-20095 | 1:250 for IF |
| Antibody | α-PITX1 (Rabbit polyclonal) | NovusBio | Cat: NBP188644 | 1:200 for IF |
| Antibody | α-LRIG1 (Goat polyclonal) | R&D Systems | Cat#: AF3688 | 1:250 for IF |
| Antibody | α-SMA (Rabbit polyclonal) | Abcam | AB5694 | 1:300 for IF |
| Antibody | α-SMA-cy3 (mouse monoclonal) | Sigma | C6198 | 1:300 for IF |
| Antibody | α-EpCAM (rat polyclonal) | BioLegend | Cat#: 118219 | 1:200 for FACS |
| Sequence-based reagent | RNAscope probe mm-Irx2 | Advanced Cell Diagnostics | Cat#: 519901 | |
| Sequence-based reagent | RNAscope probe mm-Wnt7b | Advanced Cell Diagnostics | Cat#: 401131 | |
| Sequence-based reagent | RNAscope probe mm-Pcdh10 | Advanced Cell Diagnostics | Cat#: 477781-C3 | |
| Sequence-based reagent | RNAscope probe mm-Tppp3 | Advanced Cell Diagnostics | Cat#: 586631 | |
| Sequence-based reagent | RNAscope probe mm-Ly6h | Advanced Cell Diagnostics | Cat#: 587811 | |
| Sequence-based reagent | RNAscope probe mm-Krt19 | Advanced Cell Diagnostics | Cat#: 402941 | |
| Sequence-based reagent | RNAscope probe mm-Klf5 | Advanced Cell Diagnostics | Cat#: 444081 | |
| Sequence-based reagent | RNAscope probe mm-Foxe1 | Advanced Cell Diagnostics | Cat#: 509641 | |
| Sequence-based reagent | RNAscope probe mm-Crabp1 | Advanced Cell Diagnostics | Cat#: 474711-C3 | |
| Sequence-based reagent | RNAscope probe mm-Bmp4 | Advanced Cell Diagnostics | Cat#: 401301-C2 | |
| Sequence-based reagent | RNAscope probe mm-Nrp2 | Advanced Cell Diagnostics | Cat#: 500661 | |
| Sequence-based reagent | RNAscope probe mm-Dcn | Advanced Cell Diagnostics | Cat#: 413281-C3 | |
| Sequence-based reagent | RNAscope probe mm-Has2 | Advanced Cell Diagnostics | Cat#: 465171-C2 | |
| Sequence-based reagent | RNAscope probe mm-Meis2 | Advanced Cell Diagnostics | Cat#: 436371-C3 | |
| Sequence-based reagent | RNAscope probe mm-Ackr3 | Advanced Cell Diagnostics | Cat#: 482561-C2 | |
| Sequence-based reagent | RNAscope probe mm-Axin2 | Advanced Cell Diagnostics | Cat#: 400331-C3 | |
| Sequence-based reagent | RNAscope probe mm-Shh | Advanced Cell Diagnostics | Cat#: 314361 | |
| Sequence-based reagent | RNAscope probe mm-Ptch1 | Advanced Cell Diagnostics | Cat#: 402811-C2 | |
| Sequence-based reagent | Nkx2-1-geno1 | This paper | PCR primer | 5'-CTA-GGG-AGG-CTA -GGA-ACT-CGG-3' |
| Sequence-based reagent | Nkx2-1-geno2 | This paper | PCR primer | 5'-CCG-ACC-CAC-GTA-GAG-CC-3' |
| Sequence-based reagent | Nkx2-1-geno3 | This paper | PCR primer | 5'-CTC-TTA-TCT-GGG-ATC-GCC-TGA-G-3' |
| Sequence-based reagent | Sox2-flox-geno1 | Steevens et al., 2019 | PCR primer | 5'-TGG-AAT-CAG-GCT-GCC-GAG-AAT-CC-3' |
| Sequence-based reagent | Sox2-flox-geno2 | Steevens et al., 2019 | PCR primer | 5'-TCG-TTG-TGG-CAA-CAA-GTG-CTA-AAG-C-3' |
| Sequence-based reagent | Sox2-flox-geno3 | Steevens et al., 2019 | PCR primer | 5'-CTG-CCA-TAG-CCA-CTC-GAG-AAG-3' |
| Sequence-based reagent | Cre-geno1 | Liang et al., 2005; Vauclair et al., 2005 | PCR primer | 5'-GTT-CGC-AAG-AAC-CTG-ATG-GAC-A-3' |
| Sequence-based reagent | Cre-geno2 | Liang et al., 2005; Vauclair et al., 2005 | PCR primer | 5'-CTA-GAG-CCT-GTT-TTG-CAC-GTT-C-3' |
| Commercial assay or kit | MicroChIP Diapure columns | Diagenode | Cat#: C03040001 | DNA extraction (ChIP) |
| Commercial assay or kit | Microplex Library Preparation Kit v2 | Diagenode | Cat#: C05010012 | ChIP library prep |
| Commercial assay or kit | Rneasy Micro kit | Qiagen | Cat# 74004 | RNA extraction (bulk) |
| Commercial assay or kit | SMARTer Stranded Total RNAseq kit V2 | Takara | Cat#: 634411 | Bulk RNAseq library prep |
| Commercial assay or kit | RNA 6000 Pico Bioanalyzer kit | Agilent | Cat# 5067-1513 | Bioanalyzer (RNA) |
| Commercial assay or kit | High Sensitivity DNA Bioanalyzer kit | Agilent | Cat#: 5067-4626 | Bioanalyzer (DNA) |
| Commercial assay or kit | QuBit dsDNA HS Assay kit | Invitrogen | Cat#: Q32854 | DNA quantification |
| Commercial assay or kit | Chromium Single Cell ' Reagent Kit V2 | 10X Genomics | Cat#: PN-120237, PN-120236, PN-120262 | Single cell RNA-seq |
| Other | Sytox Blue nuclei acid stain | Thermo | Cat: S11348 | Live/dead stain for FACS, use at 1uM |
| Other | TSAplus Fluoresceine | Akoya Biosciences | NEL741001KT | RNAscope, 1:1500 |
| Other | TSAplus Cy3 | Akoya Biosciences | NEL744001KT | RNAscope, 1:1500 |
| Other | TSAplus Cy5 | Akoya Biosciences | NEL745001KT | RNAscope, 1:1500 |
| Other | DynaBeads, protein G | Invitrogen | Cat#: 10003D | ChIP |
| Other | AMPure XP beads | Beckman Coulter | Cat#: A63881 | Library cleanup |
| Other | TrypLE Express | Thermo | Cat# 12604013 | Tissue dissociation |