Figure 6. Maf cDKOs overproduce INs that will become HINs.

(A) Schema depicting the BrdU/EdU pulse-chase experiment. Briefly, BrdU was injected into a pregnant mouse at E12.5 and EdU was injected at E15.5. Neonatal pups were harvested at P2 analyzed for Lhx6/BrdU, Lhx6/EdU co-expression. (B) Quantification of Lhx6⁺ IN cell density in the P2 neocortex. (C) Quantification of Lhx6⁺ IN cell density in the P2 hippocampus by region. (D–G) Lhx6 FISH with BrdU (D-E, early-born) or EdU (F-G, late-born) co-labeling in P2 WT and cDKO hippocampus. (H–I) Quantification of the Lhx6⁺; BrdU⁺ IN cell density in the hippocampus by region (H) and in total (I). (J–K) Quantification of the Lhx6⁺; EdU⁺ IN cell density in the hippocampus by region (J) and in total (K). N = 3 per group and multiple sections were quantified per animal. Scale bar in (E) = 100 um. Welch’s t test.

Figure 6—figure supplement 1. Analysis of early-born and late-born MGE-derived CINs in WT and Maf cDKO.

(A–D) Lhx6 FISH with BrdU (A-B, early-born) or EdU (C-D, late-born) co-labeling on P2 WT and cDKO neocortex. (E) Quantification of the Lhx6⁺; BrdU⁺ or Lhx6⁺; EdU⁺ IN cell density in the neocortex. (F) Quantification of the early-born and late-born IN density (cDKO/WT) in the neocortex and in the hippocampus. (G) Quantification of the early-born and late-born HIN to CIN density ratio by genotypes. Scale bar in (B) = 100 um. N = 3–4 per groups. **p<0.01; Welch’s t test.