Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
Mafbf/f | Yu et al., 2013 | Generous gift from Dr. Lisa Goodrich | |
| Genetic reagent (M. musculus) |
Maff/f | Wende et al., 2012 | Generous gift from Dr. Carmen Birchmeier | |
| Genetic reagent (M. musculus) |
Nkx2.1-Cre | Xu et al., 2008 | Jax labs #008661 | |
| Genetic reagent (M. musculus) |
Sst-IRES-Cre | Taniguchi et al., 2011 | Jax labs #013044 | |
| Genetic reagent (M. musculus) |
Ai14 Rosa26-tdTomato (RosaT) | Madisen et al., 2010 | Jax labs #007908 | |
| Antibody | Rabbit anti-PARVALBUMIN (polyclonal) |
Swant | PV27 RRID:AB_2631173 |
1:400 dilution; no need for antigen retrieval |
| Antibody | Rat anti-SOMATOSTATIN (monoclonal; clone YC7) |
Millipore Sigma | MAB354 RRID:AB_2255365 |
1:200 dilution; works well in low fixed tissue |
| Antibody | Rabbit anti-MEF2C (polyclonal) |
Proteintech | 10056–1-AP RRID:AB_513447 |
1:500 dilution; no need for antigen retrieval |
| Antibody | Rat anti-BrdU antibody (monoclonal [BU1/75(ICR1)] |
Abcam | Ab6326 RRID:AB_305426 |
1:500 dilution; BrdU was stained after FISH procedure (proteinase K-based antigen retrieval) |
| Antibody | Sheep anti-DIG-POD antibody (polyclonal) |
Millipore Sigma | 11207733910 RRID:AB_514500 |
1:1000 dilution |
| Antibody | Sheep anti-Fluorescein-POD antibody (polyclonal) |
Millipore Sigma | 11426346910 RRID:AB_840257 |
1:1000 dilution |
| Antibody | Chicken anti-GFP (polyclonal) | Aves | GFP-1020 RRID:AB_100000240 |
1:1000 dilution |
| Commercial assay or kit | Click-iT EdU kit | Life Technologies | Cat #C10340 | Follow the manufacturer’s instruction |
| Commercial assay or kit | RNAscope multiplex fluorescent kit (version 2) | ACD Bio | Cat #323120 | Follow the manufacturer’s instruction |
| Commercial assay or kit | Mm-Igfbp4 | ACD Bio | Cat #425711 | Follow the manufacturer’s instruction and used it with the RNAscope multiplex fluorescent kit (version 2) |
| Commercial assay or kit | Mm-Sst | ACD Bio | Cat #404631 | |
| Commercial assay or kit | Mm-Sp9 | ACD Bio | Cat #564531 | |
| Commercial assay or kit | Mm-Mef2c | ACD Bio | Cat #421011 | |
| Commercial assay or kit | Mm-Tcf12 | ACD Bio | Cat #504861 | |
| Commercial assay or kit | Mm-tdTomato | ACD Bio | Cat #317041 | |
| Commercial assay or kit | Mm-Pnoc | ACD Bio | Cat #437881 |