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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2019 Dec 10;15(3):554–563. doi: 10.1007/s11481-019-09895-6

Figure3. Exosome effects on BBB permeability and ability for trans-endothelial migration.

Figure3.

A) TEER measurements of the BBB pre and post incubation (48 hr) with exosomes isolated from HTHU cells alone or with exosomes isolated from HTHU cells loaded with Tspan2 siRNA in the apical side of BBB model (n=3). B) Trans-endothelial migration (24 hr) of HTHU exosomes loaded with Texas-red control siRNA to HTHU-HIV microglial cells, a) untransfected (UT) HTHU-HIV cells, b) HTHU exosomes loaded with Texas-Red control siRNA. The blue fluorescence arises from DAPI staining of nuclei, and the red fluorescence arises from Texas-Red control siRNA. Representative 20X magnification image. C) Quantification of fluorescence intensity Texas-Red control siRNA using Image J software (total of n=3 experiments).