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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2019 Dec 10;15(3):554–563. doi: 10.1007/s11481-019-09895-6

Figure 4. Therapeutic effect of exosomes on Tspan2 gene expression.

Figure 4.

HTHU exosomes loaded with Cy5 labeled Tspan2 siRNA were applied to the apical side of the BBB, 24 hr later, HTHU-HIV microglial cells on the basolateral side were imaged or analyzed for Tspan2 gene expression. A) Representative 20X images of HTHU-HIV cells following transmigration of exosomes loaded with increasing concentrations of Cy5-Tspan2 siRNA (red color). B) Corresponding quantification of cell fluorescence. C) Corresponding gene expression levels for Tspan2 quantified by q-PCR (total of n=3 experiments with 2 replicates). TAI= transcript accumulation index.