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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Gastroenterology. 2020 Feb 26;158(8):2236–2249.e9. doi: 10.1053/j.gastro.2020.02.034

Figure 3: Alterations in brush border markers persist along the length of the small intestine in MYO5B(P663L) pigs.

Figure 3:

(A) Phosphorylated ezrin-radixin-moesin (P-ERM) immunostaining showed normal apical localization in WT enterocytes. In contrast, MYO5B(P663L) enterocytes had prominent inclusions that were P-ERM positive. P-ERM immunostaining also demonstrated decreased microvilli height in MYO5B(P663L) enterocytes compared to WT enterocytes. (B) CD10 has been used to diagnose MVID in patients. CD10 immunostaining had apical localization in WT enterocytes. In MYO5B(P663L) enterocytes, CD10 was observed diffusely below the apical membrane. (C & E) Immunofluorescence staining showed P-ERM positive inclusions throughout the jejunum and ileum in MYO5B(P663L) pigs. (D & F) CD10 localized subapically in MYO5B(P663L) enterocytes in the jejunum and ileum. Scale bars=50 and 2 μm in low and high magnification respectively.