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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Gastroenterology. 2020 Feb 26;158(8):2236–2249.e9. doi: 10.1053/j.gastro.2020.02.034

Figure 4: Pig MYO5B(P663L) enteroids mimic in vivo findings.

Figure 4:

(A) Brightfield image of WT and MYO5B(P663L) 3D enteroids appeared similar. Immunofluorescence staining for P-ERM and p120 showed disorganized cell structure in MYO5B(P663L) enteroids and aberrant expression of P-ERM. (B) F-actin staining showed the presence of subapical inclusions in MYO5B(P663L) derived enteroids. (C) Schematic of the development of 2D enteroid monolayer cultured using air liquid interface. (D) Immunostaining of enteroid monolayers demonstrated well developed brush border in WT enterocytes that expressed NHE3 on the apical membrane. Cultured MYO5B(P663L) enterocytes appeared disorganized with subapical accumulation of NHE3. (E) SGLT1 immunostaining showed apical expression in many WT enterocytes. In MYO5B(P663L) enterocytes SGLT1 was observed diffusely below the apical membrane. Scale bars=50 μm in (A), 2 μm in (B), 10 μm in (C & D).