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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Gastroenterology. 2020 Feb 26;158(8):2236–2249.e9. doi: 10.1053/j.gastro.2020.02.034

Figure 6: Expression of MYO5B and MDR1 in WT and MYO5B(P663L) hepatocytes.

Figure 6:

(A) H&E of WT and MYO5B(P663L) pig liver showed the presence of lipid droplets in WT hepatocytes but no other difference was observed. Scale bars=50 μm. (B & C) In WT pigs, MYO5B appeared closely associated with the canalicular membrane, delineated by MRP2 immunostaining. MYO5B also appeared throughout the cytoplasm of hepatocytes. In MYO5B(P663L) hepatocytes MYO5B appeared in dense clusters more distant from the canalicular membrane compared to WT hepatocytes, distance of MYO5B from MRP2 is indicated by arrows. Less cytoplasmic MYO5B was observed in MYO5B(P663L) pigs compared to WT pigs. Scale bars=50 and 5 μm, respectively. (D) MDR1 in WT and MYO5B(P663L) hepatocytes demonstrated decreased expression of MDR1 in MYO5B(P663L) hepatocytes compared with WT. MDR1 appeared diffusely below the canalicular membrane in MYO5B(P663L) hepatocytes.