Fig. 6.

GSK3β inhibitors block Cdt-induced Syk phosphorylation. THP-1 ^WT-derived macrophages were pre-incubated with medium, 10μM GSK-3 Inh X or 1.0 μM GSK-3 Inh XV for 60. Medium or 200 ng/ml Cdt was then added to cell cultures and cells harvested 2 hr later. Cell extracts were analyzed for Syk and pSyk by Western blot. GAPDH was used as a loading control. Results are plotted as the relative protein levels (% control) and represent the mean±SEM of three experiments. A representative blot is shown in the inset.* indicates statistical significance relative to cells treated with medium.