Fig. 1.

Generation of Atp13a2 deficient zebrafish. (A) Sequence data for each genotype. A = green, T = red, G = black, and C = blue. The red square indicates the deletion site. (B) Semi-quantitative RT-PCR at 16, 20, and 24 cycles of atp13a2 mRNA for each genotype. (C) Real-time PCR of atp13a2 mRNA. Two types of primers were designed at different positions on the mRNA transcript. The left is the mRNA containing the deletion sites from exon 2 to exon 3, and the right is the mRNA from exon 17 to exon 18. n = 3 for each genotype. ***p < 0.001. WT indicates wild type and KO indicates Atp13a2 deficient zebrafish (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).