Figure 1.

TLR and Notch expression is downregulated in bone marrow cells of HFD-fed Gipr^−/− mice. mRNA levels of the indicated TLR and Notch signaling-related genes, relative to Ppia gene expression, were assessed in isolated BM cells from 8- to 13-week-old RCD-fed (n = 6–17/group) (A, C) and from 30- to 34-week-old HFD-fed (n = 8–14/group) (B, D) WT and Gipr^−/− male mice. (E) Gipr mRNA levels, relative to Ppia, in the indicated cell populations isolated by flow cytometry from peripheral blood, spleen, and bone marrow of 8-week-old Tie2-cre and Gipr^Tie2−/- female mice. mRNA levels of the indicated TLR and Notch signaling-related genes, relative to Ppia gene expression, were assessed in isolated BM cells from 8- to 13-week-old RCD-fed (n = 6–17/group) (F,G) and from 30- to 34-week-old HFD-fed (n = 8–14/group) (H, I) Tie2-cre and Gipr^Tie2−/− male mice. Data are presented as the mean ± SD. ∗P ≤ 0.05, ∗∗P ≤ 0.01, ∗∗∗P ≤ 0.001, and ∗∗∗∗P ≤ 0.0001. RCD: regular chow diet; HFD: high-fat diet; wks: weeks; BM: bone marrow; PB: peripheral blood; SPL: spleen; M cells: myeloid cells; HSC: hematopoietic stem cells; ST-HSC: short-term hematopoietic stem cells; LT-HSC: long-term hematopoietic stem cells; MPP: multipotent progenitor; CLP: common lymphoid progenitor; CMP: common myeloid progenitor; GMP: granulocyte-monocyte progenitor; MEP: megakaryocyte-erythroid progenitors.