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. 2020 May 7;39:101008. doi: 10.1016/j.molmet.2020.101008

Figure 2.

Figure 2

Hematopoietic responses after RCD or HFD feeding in mice transplanted with Gipr−/−donor BM. (A) Spleen weight, relative to body weight, and spleen cellularity from 26-week-old WT male mice that received WT (BMT-WT) or Gipr−/− (BMT-Gipr−/−) bone marrow at the age of 8 weeks and were fed an HFD for 14 weeks. mRNA levels of the indicated TLR and Notch signaling-related genes, relative to Ppia gene expression, were assessed in isolated BM cells from 26-week-old WT male mice that received WT (BMT-WT) or Gipr−/− (BMT-Gipr−/−) bone marrow at the age of 8 weeks and were fed an RCD (B, C) (n = 5–7/group) or HFD (D, E) (n = 4–5/group) for 14 weeks. Examination of B cells, T cells, and M cells and monocyte lineage cells (neutrophils and monocytes) at 16 weeks after BMT as a percentage of donor repopulated cells (CD45.2) in peripheral blood (F), spleen (G), and BM (H), and (I) frequency of CMP, GMP, and MEP cells in bone marrow at 16 weeks after BMT from male mice that received WT (BMT-WT) or Gipr−/− (BMT-Gipr−/−) bone marrow at the age of 8 weeks and were fed an RCD (n = 5–7/group). Examination of B cells, T cells, and myeloid cell populations (neutrophils and monocytes) at 16 weeks after BMT as a percentage of donor repopulated cells (CD45.2) in peripheral blood (J) and spleen (K), and (L) frequency of LT-ST HSC and MPP cells in bone marrow at 16 weeks after BMT from male mice that received WT (BMT-WT) or Gipr−/− (BMT-Gipr−/−) bone marrow at the age of 8 weeks and were fed an HFD for 14 weeks (n = 4–5/group). Data are presented as the mean ± SD. ∗P ≤ 0.05 and ∗∗P ≤ 0.01. BMT: bone marrow transplant; RCD: regular chow diet; HFD: high-fat diet; BM: bone marrow; SPL: spleen; PB: peripheral blood; M cells: myeloid cells; BM-HPSC: bone marrow hematopoietic progenitor stem cells; Lin-: lineage negative; ST-HSC: short-term hematopoietic stem cells; LT-HSC: long-term hematopoietic stem cells; MPP: multipotent progenitor; CMP: common myeloid progenitor; GMP: granulocyte-monocyte progenitor; MEP: megakaryocyte-erythroid progenitors.