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. 2020 May 7;39:101008. doi: 10.1016/j.molmet.2020.101008

Figure 4.

Figure 4

Hematopoietic responses to 5-FU, LPS, and Pam3CSK4 in Gipr−/−mice and WT mice treated with [DAla2]-GIP. Frequencies of B cells, T cells, M cells (A), and monocyte lineage cells (neutrophils and monocytes) (B) in bone marrow from 7-week-old WT males treated with [D-Ala]-GIP and/or 5-FU and controls (n = 6–8/group). (C) Frequency of CLP cells in bone marrow from 7-week-old WT and Gipr−/- male mice that were treated with LPS or vehicle (PBS) (n = 6/group). Frequency of LKS and LK populations (D) and CLP (E) cells in bone marrow from 7-week-old WT male mice treated with [D-Ala]-GIP and/or LPS and controls (n = 6/group). (F) Frequency of CMP, GMP, and MEP cells in bone marrow from 7-week-old WT and Gipr−/- male mice that were treated with Pam3CSK4 or vehicle (PBS) (n = 6/group). Frequencies of B cells, T cells, M cells, and monocyte lineage cells (neutrophils and monocytes) in peripheral blood (G, H) and bone marrow (I, J) from 7-week-old WT males treated with [D-Ala]-GIP and/or Pam3CSK4 and controls (n = 6/group). Data are presented as the mean ± SD. ∗P ≤ 0.05, ∗∗P ≤ 0.01, ∗∗∗P ≤ 0.001, and ∗∗∗∗P ≤ 0.0001. PBS: phosphate-buffered saline; 5-FU: 5-fluorouracil; LPS: Lipopolysaccharide; Pam3CSK4: Pam3CysSerLys4; GIP: glucose-dependent insulinotropic polypeptide; PB: peripheral blood; BM: bone marrow; BM-HPSC: bone marrow hematopoietic progenitor stem cells; LK: Lin-cKit + Sca1-; LKS: Lin-cKit + Sca1+; CLP: common lymphoid progenitor; CMP: common myeloid progenitor; GMP: granulocyte-monocyte progenitor; MEP: megakaryocyte-erythroid progenitors.