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. 2020 Jun 9;10:9299. doi: 10.1038/s41598-020-66278-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of PN and/or TNC suppresses angiogenic functions; cell proliferation, cell migration and tube formation. (A) Cell proliferation was assessed by CCK-8 assay. Effects of PN, TNC and their dual inhibition by siRNA on the cells treated with IL-13 for 24 h. Proliferation rates of HRECs are shown as fold changes. Error bars represent standard deviations. *p < 0.05, Wilcoxon rank sum test, n = 6/group. (B) Cell migration was assessed by scratch wound healing assay. Representative images of HRECs treated with PN, TNC and their dual inhibition by siRNA. The left images show the scratched cells at 0 h (Left) and 12 h (Right) after IL-13 stimulation. (C) Effects of PN, TNC and their dual inhibition by siRNA on cell migration were quantified by measuring the areas of migrated cells at 12 h after IL-13 treatment. The data are shown as fold changes. Error bars represent standard deviations. *p < 0.05, Wilcoxon rank sum test, n = 4/group. (D) Representative images of HRECs treated with PN, TNC and their dual inhibition by siRNA in Matrigel tube formation assay. (E) Data show the quantification of the total numbers of meshes and total length. Error bars represent standard deviations. ^⋆p < 0.01, *p < 0.05, Wilcoxon rank sum test, n = 6/group.