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. 2020 Apr 27;122(12):1791–1802. doi: 10.1038/s41416-020-0839-1

Fig. 6. HDAC inhibitor selectively targets mTORC1 hyper-activated tumour cells.

Fig. 6

a Western blot analysis of cleaved caspase-3 and Chop expressions upon SAHA (5 μM), SAHA + NAC (5 mM) or DMSO treatment in Tsc1iΔEC tumour cells. b, c Cell death analysis of Tsc1iΔEC tumour cells upon SAHA, SAHA + NAC or DMSO treatment. Representative results (b) and mean ± SD of MFI (c) are shown. d, e Flow cytometry analysis of ROS in Tsc1iΔEC Ctrl (shNT) and raptor knockdown (shRaptor) tumour cells upon SAHA or DMSO treatment. Cells were stained with MitoSOX Red and analysed by flow cytometry, with representative results (d) and mean ± SD of MFI (e) shown. f Western blot analysis of Chop expression in Tsc1iΔEC Ctrl (shNT) and raptor knockdown (shRaptor) tumour cells. In all western blots, vinculin is used as an endogenous loading control. For all figures, n = 3. **P < 0.01, ***p < 0.001 and ****p < 0.0001.