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. 2020 Jun 9;10:9307. doi: 10.1038/s41598-020-65787-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Post-surgical monitoring of animals following intragastric catheter implantation. (a,b) For the first 12 h following surgical catheter implantation, C57BL/6 mice were monitored every 2 h for (a) body weight and (b) pain on a scale of 0–10 (as evaluated by diminished mobility, grooming, and response to stimuli), and then thereafter every 12 h for 2 weeks. The data were combined from three independent surgical cohorts (n = 3), each performed in triplicate (9 animals total). Error bars represent standard error of the mean. (c) 2 weeks after surgical implantation of intragastric catheters, animals were euthanized, tissues were collected, paraffin embedded, and stained with hematoxylin and eosin (H&E). The site of catheter placement into the forestomach was observed by light microscopy imaging of a cross-sectional, 5 μm thick section. Images are representative of three independent surgical cohorts (n = 3) each performed in triplicate (9 animals total).