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. 2020 Jun 9;10:9365. doi: 10.1038/s41598-020-66349-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of addition of NES on initial localization and response to PKA activation. (A) Table contains the amino acid sequences of the NES that was fused to the peptide along with the derived gene names. (B) Representative images show the initial distribution (Pre) and the distribution 10 minutes post-treatment (Post) for peptides fused with NesA, NesB, NesC, and NesD. (C) Response profile shows the normalized signal change resulting from the activation of PKA for peptide fused with NesA (blue), NesB (yellow), NesC (green), and NesD (red) along with a peptide without NES (black). (D) Bar chart represents the pre-treatment localization (blue) and post-treatment localization (red) as measured by taking the ratio of cytosol-to-nuclear fluorescent signal for peptides fused with NesA, NesB, NesC, and NesD compared to a peptide without NES.All data points in this figure represent an average signal intensity calculated from 30 cells over 3 independent experiments. Error bar represents standard error of mean. * represents p < 0.001. Scale bar represents 10 µm.