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. 2020 Jun 9;10:9365. doi: 10.1038/s41598-020-66349-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Duplex monitoring of Ca2+ and PKA in cells followed by isoprenaline and FSK/IBMX treatment. (A) Ca²⁺ response as reported by R-GECO to isoprenaline (100 μM, Sigma-Aldrich, I5627-5G) and FSK/IBMX treatment in cells also expressing PKA kinase reporter and nucleus marker. (N = 3 experiments, 48 cells) (B) Nuclear-cytosol translocation response of PKA kinase reporter in same cells in (A). (C) Duplex monitoring of Ca²⁺ and PKA activity from (A) and (B).