Fig. 4. Effect of SCD1 downregulation on gastric cancer cell invasion, migration and EMT phenotype.

a Transwell migration and invasion assays demonstrated the decreased migratory and invasive abilities of SCD1-KD cells and cells treated with A939572. b Wound-healing ability was attenuated by SCD1 downregulation. c Quantification of transwell and would-healing assays. (Values represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01  and ***p < 0.001, respectively, t test.) d Immunofluorescence assays showed that downregulation of SCD1 led to an increase of E-cadherin but a decrease in vimentin and N-cadherin expression (scale bar, 10 μm in black and 25 μm in red).