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. 2020 Jun 9;11:2894. doi: 10.1038/s41467-020-16568-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Serum fibroblast growth factor 21 (FGF21) levels of Fgf21fl/fl mice at the end of an 8-week treatment with diets containing 18% from amino acids (AA; NAA) or low threonine with other AA equally adjusted to give 18% AA in total (LT); with pre-treatment with adeno-associated viruses to express Cre-recombinase (AAV-CRE) or green fluorescent protein (AAV-GFP) in an hepatocyte-selective manner. Data are mean and SEM (n = 6 NAA × AAV-GFP; n = 7 LAA AAV-GFP; n = 6 LT × AAV-CRE; n = 8 LT × AAV-CRE). Data were analysed by two-way ANOVA with Holm–Sidak post-hoc tests. Different than NAA: *P < 0.05, **P < 0.01, ***P < 0.001. Different than AAV-GFP: ^#P < 0.05, ^##P < 0.01, ^###P < 0.001. b Feed efficiency during the 8-week treatment of mice as in (a). c Energy expenditure of mice as in (a). d Insulin sensitivity index during fasting (ISI(f)) of mice as in (a).