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. 2020 Jun 9;10:9291. doi: 10.1038/s41598-020-66145-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of length and branching number of capillary-like structures observed in fibrin gels. the CD-31 immuno-labelling, length and branching number of the capillary-like structures were measured for conditions using commercially available medium EGM-2MV (EGM-2MV), unconditioned medium (DF medium), medium conditioned during 1 week by dermal fibroblasts under normoxic conditions (DF-Nx), and medium conditioned during 1 week by dermal fibroblasts under hypoxic conditions (DF-Hx). (A) Quantification of the length of the structures. N is the number of observed structures in the central field of 3 distinct gels. The length was expressed as the value provided by ImageJ software. (B) Comparison of the total length of capillary-like structures and their branching number.