Table 2.
Primer sequences used for quantitative real-time PCR.
| Primer | Primer sequence (5′ –3′) | Anneal temperature, °C | Product length, bp | GenBank accession No. |
|---|---|---|---|---|
| GAPDH | F: TGAAGGTCGGAGTGAACGGAT R: CACTTTGCCAGAGTTAAAAGCA |
55.7 | 114 | NM_001206359.1 |
| SGLT1 | F: AGAAGGGCCCCAAAATGACC R: TGTTCACTACTGTCCGCCAC |
59 | 96 | NM_001164021.1 |
| GLUT2 | F: TGGAATCAGCCAACCTGTTT R: ACAAGTCCCACCGACATGA |
55.7 | 156 | NM_001097417.1 |
| PepT1 | F: GCCAAAGTCGTCAAGTGC R: GGTCAAACAAAGCCCAGA |
63.3 | 100 | NM214347 |
| Claudin-1 | F: GCCACAGCAAGGTATGGTAAC R: AGTAGGGCACCTCCCAGAAG |
59.0 | 140 | FJ873109.1 |
| Occludin | F: CTACTCGTCCAACGGGAAAG R: ACGCCTCCAAGTTACCACTG |
59.0 | 158 | NM_001163647.2 |
| ZO-1 | F: CAGCCCCCGTACATGGAGA R: GCGCAGACGGTGTTCATAGTT |
59.0 | 114 | XM_005659811 |
| Mucin 1 | F: GTGCCGCTGCCCACAACCTG R: AGCCGGGTACCCCAGACCCA |
59.0 | 141 | XM_001926883.4 |
| Mucin 2 | F: GGTCATGCTGGAGCTGGACAGT R: TGCCTCCTCGGGGTCGTCAC |
59.0 | 181 | XM_003122394.1 |
GAPDH = glyceraldehyde 3-phosphate dehydrogenase; SGLT1 = sodium-glucose cotransporter 1; GLUT2 = glucose transporter type 2; PepT1 = oligopeptide transporter 1; ZO-1 = zonula occludens 1.