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. 2020 Mar 31;6(2):152–159. doi: 10.1016/j.aninu.2020.02.004

Table 3.

Primer and probe sequences used for quantitative real-time PCR.

Item Primer and probe sequence (5’ –3′) Anneal temperature, °C Product length, bp
Total bacteria F: ACTCCTACGGGAGGCAGCAG
R: ATTACCGCGGCTGCTGG		 57.9 200
Lactobacillus F: GAGGCAGCAGTAGGGAATCTTC
R: CAACAGTTACTCTGACACCCGTTCTTC
P: AAGAAGGGTTTCGGCTCGTAAAACTCTGTT		 53.0 126
Bifidobacterium F: CGCGTCCGGTGTGAAAG
R: CTTCCCGATATCTACACATTCCA
P: ATTCCACCGTTACACCGGAA		 57.9 121
Bacillus F: GCAACGAGCGCAACCCTTGA
R: TCATCCCCACCTTCCTCCGGT
P: CGGTTTGTCACCGGCAGTCACCT		 53.0 92
Escherichia coli F: CATGCCGCGTGTATGAAGAA
R: CGGGTAACGTCAATGAGCAAA
P: AGGTATTAACTTTACTCCCTTCCTC		 53.0 96