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. 2020 Jun 3;8:436. doi: 10.3389/fcell.2020.00436

FIGURE 5.

FIGURE 5

PT inhibits TGF-β-triggered EMT in NRK-52E cells. NRK-52E cells were treated with (A) 0, 0.5, 1, or 2.5 μM PT, or (B) 0, 2.5, 5, or 10 ng/ml TGF-β for 24 or 48 h. Cell viability were detected by MTT assay. Mean ± SD; n = 3. (C) NRK-52E cells were treated with DMSO (Control), PT 2 μM, TGF-β 2.5 ng/ml, or PT combined with TGF-β for 72 h. The morphological changes of NRK-52E cells were recorded under a phase-contrast microscopy. F-actin/ZO-1 co-staining, or Vimentin staining were determined by immunofluorescence staining. Bar = 100 μm (D) The expression of E-cadherin, fibronectin, α-SMA, and Vimentin were determined by Western blotting analysis in NRK-52E cells treated with PT, TGF-β, or PT + TGF-β. The membrane was probed with anti-GAPDH to confirm equal loading of proteins. Immunoblots are representative of at least three independent experiments.