Table 1.

ProtParam analysis for the proteins

Protein	No. of amino acids	Molecular weight	Theoretical pI	Extinction coefficients	Estimated half-life (h)	Instability index	Aliphatic index	GRAVY
PmpD	1531	160,748.22	4.75	105,795, 104,170	30	33.6	83.75	− 0.056
PmpF	1034	112,392.82	8.88	118,900, 118,150	30	33.85	76.29	− 0.284
PknD	934	107,666.41	6.24	143,895, 143,020	30	48.63	97.98	− 0.113
Yop translocation protein	921	100,580.4	5.55	56,185, 55,810	30	48.97	96.22	− 0.186