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. 2020 Jun 3;11:996. doi: 10.3389/fmicb.2020.00996

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Copyright © 2020 Mei, Jiang, Zou, Wang, Zhou, Li, Liu, Tan, Wei, Li, Dai, Peng, Zhang, Lopez-Ribot, Shapiro, Chen, Liu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased mannan and decreased chitin content after treatment with robenidine. Cells were incubated in YPD supplemented with or without robenidine (8 μM) for 4 h. Then mannan and chitin content of cell wall were measured accordingly. (A) Calcofluor White staining for cell wall chitin. (B) Surface exposed chitin stained by WGA-lectin fluorescent antibody. ****p < 0.0001. (C) Flow cytometry analysis of cell wall mannan stained by ConA-488. The detection of mannan and chitin has been repeated three times.