FIGURE 7.

Robenidine regulates cell wall integrity pathway through Rlm1. (A) WT (SN250) and rlm1 null mutant were exposed to 4 μM robenidine and OD₆₀₀ was monitored every 15 min. (B) Quantitative RT-PCR analysis of RLM1 expression after treatment of 8 μM robenidine for 90 min, 3 h, 6 h, and 9 h. Error bars indicate standard deviation based on three technical replicates. (C) Biofilm formation of WT (SN250) and rlm1 null mutant in RPMI medium supplemented with different concentrations of robenidine. Cells were incubated at 37°C for 24 h and stained with XTT solution. Photos were taken after incubation for 2.5 h. (D) Effect of robenidine on filamentous induction of WT (SN250) and rlm1 null mutant under M199 medium. Scale bar, 20 μm. Results shown are representative result of 100 cells. Each of these experiments had 3 repetitions of data.