FIGURE 1.

Analysis of expression patterns of GmWRKY142. (A) qRT-PCR analysis of the GmWRKY142 transcript in different tissues of the soybean variety Huaxia 7. mRNAs were isolated from roots, stems, leaves, apex, flowers, pods, and seeds. (B) Dose-dependent GmWRKY142 expression in roots. Roots were exposed to different Cd concentrations (0, 5, 10, 15, 25, and 50 μM) for 6 h. (C) For the time-course experiment, seedlings were exposed to 25 μM CdCl₂ for 0, 1, 2, 4, 6, 8, 12, or 24 h. Samples were separately harvested for qRT-PCR analysis. Values are expressed as the means ± SD (n = 3). The experiment was performed with at least three independent biological replicates. Significant differences according to the one-way analysis of variance are denoted as follows: *p < 0.01.