TABLE 1.
GmWRKY family genes respond to Cd stress.
Gene ID of NCBI | Gene numbers | Gene name |
Fragments Per kilobase per million (FPKM) |
Fold change (FC) | Log2(FC) | qRT-PCR | |||||
CK_1 | CK_2 | CK_3 | Cd_1 | Cd_2 | Cd_3 | ||||||
100781603 | Glyma.15G186300 | GmWRKY146 | 3.89 | 3.23 | 2.47 | 1.13 | 0.68 | 0.69 | 0.26 | –1.94 | -4.33 ± 0.23 |
100788213 | Glyma.09G129100 | GmWRKY95 | 1.27 | 1.29 | 0.91 | 0.61 | 0.10 | 0.22 | 0.27 | –1.90 | -4.89 ± 0.52 |
100783661 | Glyma.19G221700 | GmWRKY184 | 10.66 | 6.40 | 10.14 | 3.12 | 2.35 | 3.11 | 0.32 | –1.66 | -3.52 ± 0.34 |
100127370 | Glyma.01G224800 | GmWRKY7 | 2.42 | 0.97 | 0.90 | 3.14 | 5.27 | 4.05 | 2.90 | 1.54 | 2.35 ± 0.84 |
100127423 | Glyma.03G220100 | GmWRKY25 | 2.65 | 1.32 | 3.06 | 8.96 | 7.07 | 4.43 | 2.91 | 1.54 | 2.58 ± 0.09 |
100802124 | Glyma.15G110300 | GmWRKY142 | 33.19 | 30.90 | 27.40 | 76.42 | 104.34 | 92.06 | 2.98 | 1.58 | 4.02 ± 0.52 |
100127367 | Glyma.01G128100 | GmWRKY4 | 2.18 | 2.36 | 0.82 | 5.55 | 6.03 | 4.63 | 3.02 | 1.60 | 2.36 ± 0.07 |
100127421 | Glyma.11G163300 | GmWRKY114 | 11.68 | 10.37 | 13.61 | 31.62 | 46.88 | 34.56 | 3.17 | 1.66 | 3.67 ± 0.10 |
100170682 | Glyma.18G213200 | GmWRKY172 | 0.12 | 0.48 | 1.41 | 2.66 | 1.44 | 2.53 | 3.30 | 1.72 | 2.48 ± 0.12 |
100797998 | Glyma.12G097100 | GmWRKY115 | 0.93 | 0.67 | 0.12 | 1.93 | 2.32 | 1.74 | 3.48 | 1.80 | 3.47 ± 0.25 |
100127387 | Glyma.03G256700 | GmWRKY28 | 1.32 | 0.41 | 0.61 | 2.45 | 3.76 | 2.27 | 3.61 | 1.85 | 3.41 ± 0.33 |
100807966 | Glyma.07G023300 | GmWRKY68 | 3.93 | 2.48 | 5.31 | 12.84 | 13.75 | 15.90 | 3.62 | 1.86 | 4.08 ± 0.41 |
732588 | Glyma.15G003300 | GmWRKY141 | 3.02 | 3.59 | 4.41 | 11.78 | 16.75 | 15.86 | 4.03 | 2.01 | 3.59 ± 0.53 |
100795177 | Glyma.06G307700 | GmWRKY66 | 1.85 | 2.13 | 1.23 | 7.68 | 8.35 | 7.12 | 4.44 | 2.15 | 4.13 ± 0.22 |
102666898 | Glyma.03G042700 | GmWRKY21 | 2.34 | 2.68 | 1.48 | 8.34 | 11.75 | 11.64 | 4.88 | 2.29 | 4.53 ± 0.84 |
100127375 | Glyma.08G021900 | GmWRKY78 | 0.58 | 0.85 | 0.58 | 2.10 | 4.09 | 4.09 | 5.13 | 2.36 | 5.55 ± 1.19 |
100787050 | Glyma.05G127600 | GmWRKY44 | 0.29 | 0.36 | 0.26 | 1.82 | 1.36 | 1.82 | 5.47 | 2.45 | 5.58 ± 0.98 |
100170747 | Glyma.16G026400 | GmWRKY147 | 0.87 | 0.77 | 0.70 | 5.04 | 7.62 | 4.47 | 7.31 | 2.87 | 8.22 ± 1.14 |
100798500 | Glyma.04G061400 | GmWRKY31 | 0.12 | 0.20 | 0.15 | 1.74 | 1.39 | 1.01 | 8.89 | 3.15 | 8.47 ± 1.21 |
100790050 | Glyma.19G254800 | GmWRKY185 | 0.17 | 0.45 | 0.74 | 4.05 | 4.74 | 4.00 | 9.41 | 3.23 | 10.77 ± 1.49 |
100779533 | Glyma.18G092200 | GmWRKY168 | 0.27 | 0.77 | 0.38 | 4.28 | 5.13 | 4.97 | 10.13 | 3.34 | 12.36 ± 1.37 |
100816891 | Glyma.08G082400 | GmWRKY81 | 0.32 | 0.65 | 0.28 | 4.53 | 5.10 | 5.47 | 12.11 | 3.60 | 14.56 ± 2.56 |
100812027 | Glyma.06G125600 | GmWRKY56 | 0.21 | 0.48 | 0.09 | 3.03 | 3.24 | 3.82 | 12.94 | 3.69 | 15.21 ± 1.74 |
100127378 | Glyma.08G011300 | GmWRKY76 | 0.06 | 0.27 | 0 | 0.31 | 2.27 | 1.73 | 13.30 | 3.73 | 13.97 ± 1.55 |
100127373 | Glyma.06G061900 | GmWRKY53 | 0.02 | 0.21 | 0.22 | 2.05 | 2.66 | 1.68 | 13.97 | 3.80 | 15.20 ± 2.48 |
100776906 | Glyma.04G238300 | GmWRKY39 | 0.05 | 0.06 | 0.03 | 0.31 | 0.67 | 1.38 | 18.34 | 4.20 | 20.24 ± 3.0 |
102667019 | Glyma.01G222300 | GmWRKY6 | 0.05 | 0.11 | 0 | 0.36 | 2.24 | 1.54 | 26.11 | 4.71 | 27.55 ± 2.76 |
100798375 | Glyma.13G370100 | GmWRKY126 | 0.03 | 0.07 | 0.28 | 2.26 | 4.43 | 4.22 | 28.21 | 4.82 | 28.42 ± 2.45 |
732586 | Glyma.19G094100 | GmWRKY180 | 1.04 | 0.48 | 0.64 | 20.04 | 30.36 | 28.53 | 36.42 | 5.19 | 44.87 ± 5.64 |