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. 2020 Jun 3;10:878. doi: 10.3389/fonc.2020.00878

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Copyright © 2020 Sun, Yang, Wang, Zhang, Jin, Xie, Jin, Pang, Zhao, Lu, Sang, Zhang, Lin, Sun, Huang and Mao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell survival and proliferation in the 3D bioprinted liver cancer cell model. (A) Cell viability at different times after printing. Representative live-dead staining images of 3DP-HepG2 structures at days 1, 3, 5, 7, and 10 after printing. Live and dead cells were labeled with calcein-AM (green) and PI (red), respectively. Scale bar: 300 μm. Histogram of cell viability at different times after printing (B) Proliferation rates of 3DP-HepG2 and 2D-HepG2 cells at different time points.