FIG 4.

Expression of several ScmR-regulated genes in cultures of B. thailandensis E264 wild-type and scmR mutant strains buffered or not buffered with HEPES. The relative transcript levels of obc1 (A), BTH_I3204 (B), bsaN (C), BTH_II0639 (D), btaR5 (E), and BTH_II1209 (F) were assessed by qRT-PCR in cultures of B. thailandensis E264 wild-type and scmR mutant strains. Cultures were buffered with 100 mM HEPES. Water only was added to the controls. The results are presented as relative quantification of transcription of the gene compared to the wild-type strain, which was set at 100%. The error bars represent the SD of the averages for three replicates.