TABLE 1.
HutC target sites whose activity was confirmed in vitro in this work
| Site sequenceb | Locationa | Locus tag | Gene product and function |
|---|---|---|---|
| TGTATATACA | ← • → | Pflu0848 | PlcC, phosphatidylcholine-hydrolyzing phospholipase C |
| Pflu0849 | 5-Dehydro-4-deoxyglucarate dehydratase | ||
| TGTATAAACA | ← • → | Pflu2466 | Hypothetical protein |
| Pflu2467 | AraC family transcriptional regulator | ||
| CGTATATACA | ← • → | Pflu0460 | AceE, pyruvate dehydrogenase subunit E1 |
| Pflu0461 | GlnE, bifunctional glutamine synthetase adenylyltransferase/deadenyltransferase | ||
| TGTCCGAACA | ← •← | Pflu0344 | NtrB, sensor kinase for nitrogen metabolism |
| Pflu0345 | Putative lipoprotein | ||
| TGTATGCACA | → • → | Pflu0325 | Transporter in major facilitator family |
| Pflu0327 | HisB, imidazoleglycerol-phosphate dehydratase in histidine biosynthesis | ||
| TGTATGCACA | ←• → | Pflu6087 | AmiA, N-acetylmuramoyl-l-alanine |
| Pflu6088 | Putative GTP cyclohydrolase | ||
| CGTTTGTACA | ← • ← | Pflu4947 | PilZ, type IV pilus assembly protein |
| Pflu4948 | Putative lipoprotein | ||
| TGTATATACG | ← • → | Pflu2987 | TnpT, putative cointegrase resolution protein |
| Pflu2988 | TnpS, cointegrase |
a
HutC target site (circle) is shown together with genes located immediately upstream and downstream in the genome of P. fluorescens SBW25.
b
Putative Phut half sites are underlined.