FIG 7.

pH 7.5 is a positive signal for ltrR expression. Shown are transcriptional profiles of S. Typhi IMSS-1 harboring pKK8/ltrR2-471+90 grown in N-MM (Tris-HCl at 200 mM) at pH 6.0, 6.5, 7.0, and 7.5 (A) and the fusions pKK8/ltrRP2-471-1 and pKK8/ltrRP1+7+90 evaluated in N-MM (Tris-HCl at 200 mM) at pH 7.0 or 7.5 (B). Transcriptional activities were determined at an OD₅₉₅ of 0.6. The values are the means ± standard deviations from two independent experiments performed in duplicate (n = 4). Statistically different values are indicated (****, P < 0.0001).