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. 2020 Jun 3;14:24. doi: 10.3389/fncir.2020.00024

Figure 2.

Figure 2

Baseline transmission at medial perforant path to granule dentate gyrus (MPP-DG) synapses of Tg and WT mice. (A) Schematic of the DG recording (Rec) electrode to detect fEPSPs upon stimulation (stim) of the MPP of hippocampal brain slices. Light was delivered for photoactivation (blue light, 480 ± 15 nm) of PAC expressed within granule neurons (magenta). (B) Input/output relationship within MPP-DG synapses in the absence (WT) and presence of PAC (Tg; WT n = 7 slices/3 mice, Tg n = 16 slices/9 mice). Inset shows sample I/O responses superimposed. (C) Comparison of the paired-pulse ratio (PPR) calculated from the ratio of the second fEPSP slope to the first, at different interpulse intervals (WT n = 12 slices/4 mice, Tg n = 15 slices/7 mice). (D) Quantification of synaptic response with/without PAC photoactivation for 60 s during the baseline recording (Tg n = 11 slices/7 mice, WT n = 13 slices/3 mice). NS, p > 0.05 (unpaired t-test). Data are presented as mean ± SEM. Upper: sample paired-pulse traces show the fEPSP responses before (Baseline) and 1 min after (+Light) light stimulation. Black arrows indicate the time of the single pulses of stimulation.