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Widefield fluorescence micrographs of RAW 264.7 cells incubated with ACS-HNE/BSA (20/100 μM) before (–) or after (+) addition of human NE (HNE, 4 ng μL−1, 100 μL); Excitation and emission wavelengths for ACS-HNE are 460–490 nm and 500–550 nm, respectively. The inset shows magnified sections of the corresponding fluorescence micrograph.
