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. 2020 Jun 3;11:1117. doi: 10.3389/fimmu.2020.01117

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Copyright © 2020 Meghraoui-Kheddar, Barthelemy, Boissonnas and Combadière.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD43 expression identifies circulating and tissue resident NCMo subsets. Classical (blue gate), intermediate (red gate), and non-classial (green gate) monocyte subsets were gated according to Ly6C and CD43 markers expression in the bone marrow, the blood, the spleen, and the lungs before (A) and 4 h after LPS intraperitoneal injection (B). The distribution of monocytes subsets between the vasculature and the organs parenchyma was evaluated by blood tissue partitioning (intravascular CD45 staining) before (C) and 4 h after LPS intraperitoneal injection (D). Nr4a1 reporter expression (using Nr4a1^+/EGFP mouse was presented in each dot plot in a color scale going from blue to red (A–D). Dot-plots are representative of n = 3 per group.