Table 1.

Nucleotide sequences of primer pairs used in real-time PCR.

Gene	Accession no.	Primer position	Primer sequence	Amplicon Size, bp	References
P2Y2 receptor	NM_001302347	618–638 756–773	GCGTTTCCTCTTCTACACCAA ACCAGCACCCACACAACC	155	Designed
LPL	NM_008509	583–604757–777	AAGCTGGTGGGAAATGATGTGG TTCTGCATACTCAAAGTTAGG	194	(36)
CD36	NM_001159558	794–814 707–726	GATGACGTGGCAAAGAACAG TCCTCGGGGTCCTGAGTTAT	107	(37)
HSL	NM_010719	1083–1103 1132–1150	GGAGCACTACAAACGCAACGA TCGGCCACCGGTAAAGAG	67	(38)
ATGL	NM_001163689	724–743 849–868	AACACCAGCATCCAGTTCAA GGTTCAGTAGGCCATTCCTC	144	(39)
Insulin receptor	NM_001330056	3675–3709 3794–3816	TGAGTCAGCCAGTCTTCGAGAA GCCATCAGTTCCATCACTACCA	141	Primer finder
GLUT4	NM_001359114	429–448 482–501	GATTCCATCCCACAAGGCAC TCATGCCACCCACAGAGAAG	72	(38)
IRS1	NM_010570.4	1117–1131 1159–1177	GAGCGCCCCCAAACG GTCAGCCCGCTTGTTGATG	60	(38)
IRS2	NM_001081212	3214–3233 3285–3302	CAAGAGCCCTGGCGAGTACA CCGCGGATGCCAGTAGTG	88	(38)
TNFα	NM_013693	533–556 435–456	AAGCCTGTAGCCCACGTCGTA GGCACCACTAGTTGGTTGTCTTTG	121	(40)
MCP-1	NM_011333	214–236 404–423	CCCAATGAGTAGGCTGGAGA GCTGAAGACCTTAGGGCAGA	190	(40)
CCR2	NM_009915	36–56 152–174	GCAAGTTCAGCTGCCTGCAA ATGCCGTGGATGAACTGAGGTAA	138	(40)
CD68	NM_001291058	712–736 788–808	CATCAGAGCCCGAGTACAGTCTACC AATTCTGCGCCATGAATGTCC	96	(40)
F4/80	NM_010130	1877–1899 1989–2011	GAGATTGTGGAAGCATCCGAGAC GATGACTGTACCCACATGGCTGA	134	(40)
β-Actin	NM_031144.2	18–37 205–224	CACCCGCGAGTACAACCTTC CCCATACCCACCATCACACC	207