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. 2020 Jun 3;13:81. doi: 10.3389/fnmol.2020.00081

Figure 1.

Figure 1

Both Glycogen synthase kinase-3α (GSK-3α) and GSK-3β are activated selectively in 1-methyl-4-pheny-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Mice were treated with 1, 3, and 5 doses of MPTP and sacrificed 6 h post-injection (M1×, M3×, M5×). (A,B) Immunofluorescent detection of p-GSK-3α (Ser21; green) in TH-positive dopaminergic neurons (red) by immunofluorescence (A) and quantitative data are shown (B). Scale bar: 50 μm. Data are expressed as mean ± SEM (n = 4–5 per group). ***p < 0.001 vs. saline-treated mice. (C,D) The detection of p-GSK-3β (Ser9; green) in TH-positive dopaminergic neurons (red) by immunofluorescence (C) and quantitative data are shown (D). Scale bar: 50 μm. Data are expressed as mean ± SEM (n = 4–5 per group). ***p < 0.001 vs. saline-treated mice.