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. 2020 Jun 3;13:81. doi: 10.3389/fnmol.2020.00081

Figure 3.

Figure 3

Dopaminergic neuron-associated GSK-3β contributes to MPTP-induced parkinsonian neurodegeneration. (A) Immunofluorescence analysis for GSK-3β (green) in the ventral midbrains from MPTP-injected littermate control and Gsk3bΔDat mice. TH (red) staining marks dopaminergic neurons in the substantia nigra. Scale bar: 50 μm. n = 3 per group. (B,C) Immunohistochemical staining of TH on the midbrain sections from saline- and MPTP-injected control littermates and Gsk3bΔDat mice (A) and cell counts of TH-positive neurons of the whole SNpc are shown (C). Scale bar: 100 μm. Data show the mean ± SEM (n = 5–6 per group). *p < 0.05, ***p < 0.001 vs. saline-treated mice of the same genotype. #p < 0.05 vs. control littermates as indicated. (D,E) Nissl staining on the midbrain sections from saline- and MPTP-injected control littermates and Gsk3bΔDat mice (D) and cell counts of Nissl-positive neurons of the whole SNpc are shown (E). Scale bar: 100 μm. Data show the mean ± SEM (n = 5–6 per group). **p < 0.01, ***p < 0.001 vs. saline-treated mice of the same genotype. ##p < 0.01 vs. control littermates as indicated. (F,G) Immunohistochemical staining of TH on striatal sections from saline- and MPTP-injected control littermates and Gsk3bΔDat mice (F) and measured IOD of striatal TH immunostaining (G). Scale bar: 500 μm. Data show the mean ± SEM (n = 5–6 per group). *p < 0.05, ***p < 0.001 vs. saline-treated mice of the same genotype. #p < 0.05 vs. control littermates as indicated.