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. 2020 Jun 3;11:563. doi: 10.3389/fphys.2020.00563

FIGURE 1.

FIGURE 1

EPA, DPA, and DHA protect against PAL-induced reductions in cell viability. Trypan blue exclusion test dose response experiment in myoblasts following 16 h of palmitate (PAL) treatment to assess cell viability (A). Trypan blue exclusion test of cell viability following treatment with 500 μM PAL and/or 50 μM EPA, DPA, or DHA (B). Representative images of nuclear DAPI staining following treatments (C). Average number of nuclei/field (D), brightness/nuclei (E), nuclear area (F), and nuclear perimeter (μm) (G). Treatments that do not share letters are considered statistically significant (p < 0.05; myoblasts were cultured and harvested independently to generate a total sample size of 3–4 biological replicates). Scale bar represents 125 μm. Values are presented as mean ± SEM.