FIGURE 6.

Treatment with DPA and/or PAL does not alter myoblast mitochondrial content. Protein content of mitochondrial OXPHOS complexes following 16 h treatment with 500 μM PAL and/or 50 μM EPA, DPA, or DHA and representative images (A–F). All densitometry values are expressed relative to protein content determined by stain free imaging (A; representative image shown with molecular weight markers indicated) and presented in arbitrary units relative to CON. Myoblasts were cultured and harvested independently to generate a total sample size of 6 biological replicates. Values are presented as mean ± SEM.